Characterization of a second gene product related to rabbit cytochrome P-450 1.
نویسندگان
چکیده
منابع مشابه
ISOLATION AND PARTIAL CHARACTERIZATION OF THE GENE FOR CYTOCHROME P-450 3a (P-450~~~) AND A SECOND CLOSELY RELATED GENE Shahrokh
Two genes that hybridize to the cDNA for alcoholinducible cytochrome P-450 form 3a (P-450ALC 1 have been isolated from a rabbit genomic library and characterized by restriction mapping, hybridization, and partial sequence analysis. The genes show extensive sequence similarity as judged by hybridization at high stringency to the coding region of P-450 3a cDNA. However , only gene 1 hybridizes un...
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Cytochrome P-450 was purified to apparent homogeneity from the brain microsomes of phenobarbital-treated rats. The specific content of the purified P-450 was 12.7 nmol/mg of protein. NADPH-cytochrome P-450 reductase (reductase) was also purified to apparent homogeneity from brain microsomes. The specific content was 34.7,mol of cytochrome c reduced/min per mg of protein. The reduced carbon mono...
متن کاملGene conversion in a cytochrome P-450 gene family.
The mRNAs encoding the two major phenobarbital-inducible forms of cytochrome P-450 of rat liver, P-450b and P-450e, are remarkably similar (98% homologous) in nucleotide sequence, but the distribution of differences within them is not random. While the 5' halves (approximately equal to 1 kilobase) appear to be identical, there are 36 divergent residues in the remaining sequences of the two mRNA...
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Since the state of biological hydroxylation was perceptively summarized in fact and projection by Boyd (1972), extraordinary progress has been made both in the isolation of a variety of the mammalian enzymes from liver and adrenal tissues (Takemori et al., 1975; Imai & Sato, 1974; Van der Hoeven et al., 1974) and in the establishment of the nature of oxygen intermediates and the role and site o...
متن کاملNADPH: cytochrome P-450 reductase in olfactory epithelium. Relevance to cytochrome P-450-dependent reactions.
The presence of a very active cytochrome P-450-dependent drug-metabolizing system in the olfactory epithelium has been confirmed by using 7-ethoxycoumarin, 7-ethoxyresorufin, hexobarbitone and aniline as substrates, and the reasons for the marked activity of the cytochrome P-450 in this tissue have been investigated. The spectral interaction of hexobarbitone and aniline with hepatic and olfacto...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1987
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)45662-1